报告题目：Gold Nanoparticle-Polyplex Electroporation in the Enhancement of Nucleic Acid Delivery
报告时间：2018年6月15日 15:00——16：00
报告地点：实验一楼第一会议室
联系人：刘润辉教授
报告人介绍：
Short biography
2011 Harrelson Family Endowed Professorship
2014 Associate Professor, Louisiana Tech University
2008 Assistant Professor, Louisiana Tech University
2007 Research Engineer, NSF Nanoscience and Engineering Center for Affordable Nanoengineering of Polymeric Biomedical Devices (CANPBD)
2006 PhD, The Ohio State University
2000 M.S, Dalian Institute of Chemical Physics
1997 B.S, Zhejiang University
Honors & Awards
2011 Faculty Outstanding Research Achievement Award, LA Tech
Abstract
Nonviral nanoparticles cannot yet become competitive over natural virus-based counterparts in many therapy strategies, which ties with their poor nanoparticle quality, slow and ineffective endocytosis-mediated cellular uptake, and undesired cytotoxicity. We develop new AuNP-Polyplex Electroporation based new delivery route, in which (1) monodispersed gold nanoparticles (AuNPs) are used to provide controlled assembly domain and environment with precise management on molecule interactions to obtain homogeneous polyplex in size, structure, and component quantity; (2) AuNPs also help fix free and dissociated cationic polymer to reduce their cytotoxicity and enhance electroporation based delivery route; (3) electroporation is adopted to bypass the slow and inefficient endocytosis process and to promote direct cytosolic delivery and quick nuclear entry. The delivery efficiency is evaluated with K562 cells, showing 1.5~2 folds improvement on the transfection efficiency and no significant increase of toxicity when compared to free plasmid delivery by electroporation alone. The computational results are further compared to the experimental observations. With wise control strategy on the assembly quality, we anticipate such combination of physical and chemical delivery concept may stimulate further exploration in the delivery of various therapeutic materials for both in vitro and in vivo applications in vaccine or reprogramming type of applications.
AIChE J. 2018, 64, 810-821. Electrophoresis2014, 35, 1837-1845. J. BiomediNanotech 2014, 10, 982-992.